Nanozymes-Based Nucleic Acid Detection Services

Nucleic acid molecules, which carry genetic information, are the determinants of species continuity and evolution. They are related to biological growth, development, aging and carcinogenesis. Nucleic acid detection methods are mainly divided into two categories, one is based on direct nucleic acid hybridization, the other is based on nucleic acid amplification in vitro. The unique physicochemical, optical and biocompatibility of nanozymes enables them to be widely used in the field of biological detection, which brings technological innovation for nucleic acid detection.

Creative Enzymes is a leading provider of comprehensive, high-quality services for nanozymes. We provide services to customers worldwide in the field of nucleic acid detection with nanozymes. Our platform equipped with state-of-the-art facilities and highly experienced staffs in order to assist in all areas of nanozyme projects. We guarantee credible results for our customers all over the world.

Detection of DNA hybridization with Nanozymes

• Au nanozymes possess GOx-like properties, and they have a high affinity to single-stranded DNA (ss-DNA) through the gold-sulfur bond, which limits their GOx-like catalytic ability. Compared with ss-DNA, the binding capacity between double-stranded DNA (ds-DNA) and Au nanozymes is relatively weak. Au nanozymes can still retain high enzymatic activity in the presence of ds-DNA.

Fig. 1 Nanozyme-based probe for the detection of DNA hybridization.

• Combining the enzymatic capacity and the affinity between ss-DNA and ds-DNA of Au nanozymes, Creative Enzymes develops a novel nano plasmonic probe to detect DNA hybridization.

Detection of SNPs with Nanozymes

• Since graphene also exhibits different affinities for ss-DNA and ds-DNA, graphene can be used to effectively distinguish between ss-DNA and ds-DNA. In addition, the hemin component has a unique peroxidase-like character.
• Inspired by these features, Creative Enzymes designs and synthesizes of hemin-graphene hybrid nanozymes through the π−π interaction. Combination with the characteristics of hemoglobin and grapheme, the obtained hybridized nanozymes can achieve colorimetric detection of SNP.

Detection of Sequence-Specific Nucleic Acids with Nanozymes

In situ growth of Au nanozymes on graphene nanosheets, we engine a novel hybrid nanozyme to achieve the colorimetric detection of different targets such as sequence-specific nucleic acids.

Detection of DNA concentration with Nanozymes

• Based on the effect of DNA template sequence on the fluorescence intensity of silver nanozymes, we design a label-free molecular beacon for detecting target DNA concentration.
• The change of fluorescence intensity is proportional to the concentration of target DNA and has a good linear relationship in a certain range. The target DNA detection method based on silver nanozyme has the advantages of low cost, simple operation, high sensitivity and high selectivity, and can also avoid the complicated steps of labeling dye.

Creative Enzymes provides fast turnaround, high quality services at competitive prices for customers worldwide. Our advanced technical platforms can help our clients solve the problems they may encounter in research based on years of experience. If you are interested in our services, please contact us for more details or make an online inquiry.