Nanozymes-Based Immunoassay Services

Since the first report in the 1950s, nanozymes-based immunoassays with good specificity have got extensive applications in clinical diagnosis, food safety and environmental monitoring. However, the use of these natural enzymes brings some undesired defects to traditional ELISAs, including high cost and short shelf life. Fortunately, the discovery of nanomaterials with enzyme-like catalytic features (nanozymes) provides a potential way to overcome the above shortcomings. By replacing natural enzymes with artificial nanozymes, the developed immunosensors exhibit lower costs, better stability, and easier production, along with comparable sensitivity and selectivity.

Creative Enzymes provides a full range of services to our customers worldwide, from immunoassay for bioanalysis to immunohistochemical staining based on nanozymes. As a pioneer and the undisrupted global leader in nanozymes engineering and applications, we proudly introduce our professional immunoassay services as well as our diverse technologies.

Fig. 1 Comparison of (A) natural enzyme-based immunoassays and (B) nanozyme-based immunoassays.

Immunoassay for Bioanalysis with Nanozymes

• Bioassays by immunoassay allow detection without any analyte enrichment, purification, or pretreatment, which is often required for standard methods such as high-performance liquid chromatography (HPLC), mass spectrometry, or gas chromatography.
• Especially in clinical diagnosis, complex samples, such as whole blood, serum or urine, contain a large number of interfering factors including DNA, protein, amino acid, sugar, hormones, etc. Therefore, immunoassay is significantly better than standard methods in terms of simplicity and sensitivity.
• Many kinds of nanozymes can be used for immunoassay for bioanalysis, such as Fe₃O₄, Au, Pd / Pt, Cu. Creative Enzymes enables the bioanalysis of immunoassays through a variety of pathways, for example, colorimetric immunoassays, fluorescent immunoassays, chemiluminescent immunoassays, electrochemical immunoassays, multimodal immunoassays and others.

Immunohistochemical Staining with Nanozymes

• Nanozymes usually have the characteristics of antioxidant enzymes, such as peroxidase, superoxide dismutase and catalase, which can catalyze the oxidation of colorimetric substrates and generate color reactions. Nanozymes can be used to visualize biomarkers identified in tissue sections for pathological disease diagnosis and treatment monitoring.
• Creative Enzymes applies immunohistochemical staining with nanozymes for pathological diagnosis of tumors. In addition, we also apply them to the pathological identification of human high-risk and ruptured atherosclerotic plaques.
• By comparison with the traditional immunohistochemistry, the nanozyme-based pathological staining method is more rapid and sensitive because of their higher catalytic activity than natural enzymes, which greatly shortens the diagnostic time and reduces the cost and thus has significant implications.
• To further improve the detection sensitivity of nanozyme-based pathological staining method, we expend much effort to improve the enzyme-like catalytic activity of nanozymes, including adjusting their size, shape, composition, surface modification, and heteroatomic doping.

Creative Enzymes has been dedicated to nanozymes research for many years. We'll finish your studies on time and on budget. We guarantee the confidentiality and sensitivity of our customers' data. If you are interested in our services, please contact us for more details or make an online inquiry.