Nanozymes for Detection of Veterinary Drug Residues

Veterinary drugs are substances, including medicinal feed additives, that are used to prevent, treat and diagnose animal diseases or to deliberately regulate animal physiology. Veterinary drugs used on food-producing animals can create residues in eggs, milk, meat. These residues may contain compounds and metabolites that enter the body through the food chain and cause direct toxic effects. According to their functions, veterinary drugs can be divided into different classes such as antibiotics, anthelmintics, growth promoters, antigenic, trypanosomiasis drugs, sedatives, β-adrenergic receptor blockers and so on.

The abuse of veterinary drugs not only directly harms human health, but also the development of animal husbandry and the ecological environment. Therefore, it is essential to develop effective and fast pathways to screen for veterinary drug residues in food samples. CD BioSciences provides nanozymes and detailed protocols for the detection of veterinary drug residues.

Analysis of Antibiotics with Nanozyme

• Antibiotics can directly interact with nanozymes to regulate their catalytic activity.
• Based on this, we construct a colorimetric sensor based on Fe₃O₄ nanozymes for the determination of tetracycline antibiotics. Tetracycline antibiotic molecules have a strong affinity for Fe²⁺ and Fe³⁺ on the surface of Fe₃O₄, and the resulting complex can catalyze the oxidation of TMB by H₂O₂. This method can detect oxytetracycline, tetracycline and doxycycline.

Fig. 1 Colorimetric immunoassay principle for chloramphenicol based on AgI / TiO₂ tags.

• We prepare modified AgI / TiO₂ nanozymes with excellent photocatalytic activity. Consequently, a photo-responsive colorimetric immunoassay for chloramphenicol was developed by magnetic bead-based labeling of chloramphenicol and competitive binding of chloramphenicol to antibodies. In addition, the sensor requires only the addition of the substrate TMB without the use of H₂O₂, and it has high sensitivity, good specificity and good repeatability.

Analysis of Antibacterialwith Nanozyme

• Antibacterial are a class of drugs that treat or prevent infectious animal diseases by inhibiting or killing pathogenic bacteria. However, misuse of these drugs can lead to harmful effects on human health and the environment. Therefore, various analytical methods have been developed for antimicrobial analysis.

Fig. 2 Schematic representation of the biomimetic enzyme-linked immunosorbent assay procedure.

• Based on Au nanozymes, we propose a peroxisome like activity enhancement assay for norfloxacin. In addition, a biomimetic assay for immunosorbent detection of sulfadiazine using Au / SiO₂ showed good stability.

Analysis of Other Drugs with Nanozyme

• Other types of veterinary drugs such as antivirals and hormones can also easily contaminate animal food.
• We develop a colorimetric immunoassay for the detection of amantadine by introducing Pt as a nanozyme marker. The proposed method significantly improves both macroscopic and optical measurements compared to conventional signal-to-noise immunoassay.

CD BioSciences provides nanozyme and veterinary drug residue detection solutions to our clients worldwide. With years of experience in biological services, our advanced platform can help our clients address the types of difficulties associated with the detection of glutathione using nanozymes. If you are interested in our services or have some questions, please feel free to contact us or make an online inquiry.