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Nanozymes for Detection of Other Pollutants
Nanozymes can also be used in the detection of other contaminants in food, such as Sudan Red I, melamine, clenbuterol, ractopamine and other prohibited additives, hydroquinone, p-nitrophenol and other organic pollutants, NO2-, CN- and other inorganic substances.
Food safety problems caused by the misuse of prohibited additives are frequently reported. Creative Enzymes provides nanozymes and detailed schemes for detection of foodborne pathogens. Our platform is equipped with state-of-the-art facilities and highly experienced staff to assist in all areas of enzyme programs. We guarantee to deliver results on time for our customers.
| Detection Subjects | Nanozymes | Mimicking activity | Detection Method | Tested sample |
|---|---|---|---|---|
| Sudan red I | Graphene / Pt | Oxidase | Electrochemistry | Chili sauce / Tomato sauce |
| Melamine | Au | Peroxidase | Colorimetric method | Milk / Milk powder |
| Clenbuterol | (NH4)5PV8Mo4O40 / ZrO | Oxidase | Electrochemistry | Pork |
| Hydroquinone | CEVO4 | Peroxidase and Oxidase | Colorimetric method | Tap water |
| Nitrite | C / Au | Oxidase | Electrochemistry | Lake water |
| Iodide ions | Pd | Peroxidase | Colorimetric method | Tap water |
Analysis of Prohibited Additives with Nanozyme
- Sudan Red I is strongly carcinogenic and is strictly prohibited from being added to food. However, it is still widely used by criminals for food production due to its bright color, non-fading properties and low price.
- Creative Enzymes fabricates Graphene / Pt nanozyme composites. They were used to develop a sensitive and reliable novel electrochemical sensor for the determination of Sudan Red I in foods.
Fig. 1 Schematic representation of the fabrication of the graphene / Pt nanozyme composite and the electrochemical mechanism of the redox behavior of Sudan I on the nanozymes.
- Melamine can increase the detection value of protein, but its long-term intake can lead to bladder and kidney stones in the urinary system, even induce bladder cancer.
- It is found that melamine can enhance the peroxidase-like activity of Au nanozymes by inducing aggregation effect. Based on this principle, by coupling to the TMB- H2O2 reaction, we have developed a colorimetry sensor that can be observed with the naked eye and has been successfully applied to the detection of melamine.
Analysis of Organic Pollutantswith Nanozyme
- Exogenous phenolic compounds have been detected in food and environmental samples, and some are listed as carcinogens, which cannot be ignored for human health.
- We develop CEVO4 nanozymes with both peroxisome-like and oxidase activities that catalyze TMB color development. Since hydroquinone is a strong free radical resistant compound that can inhibit the CEVO4-TMB catalytic oxidation process, this colorimetric method can successfully distinguish hydroquinone with good selectivity, specificity and sensitivity.
Analysis of Inorganic Substanceswith Nanozyme
- Nitrite is widely found in nature, and it can also be used as a preservative and color protectant in meat products. However, consumption of foods high in nitrite can cause respiratory failure and even induce cancer.
- We prepared C / Au nanozymes by in-situ reduction of chlorogold acid on the surface of carbon spots for electrochemical detection of nitrite. Due to the synergistic effect of carbon spots and AuNPs, the oxidation of nitrite by C / Au nanozymes shows good electrostatic activity, enabling rapid and highly sensitive detection of nitrite in water samples.
Creative Enzymes has extensive experience and expertise in nanozyme services. We are committed to providing you with timely, quality deliverables. At the same time, we guarantee the cost-effectiveness, completeness and simplicity of the report. If you are interested in our services or have some questions, please feel free to contact us or make an online inquiry.