Nanozymes for Detection of Mycotoxins

Mycotoxins are secondary metabolites produced by fungi under suitable conditions and are major contaminants in food and feed. Due to the widespread presence of the fungus, mycotoxin contamination cannot be completely eliminated, although it can be controlled during crop growth, post-harvest storage and processing. Studies have shown that even low doses of mycotoxins can cause liver, kidney or neurological toxicity and even death. To protect human health, in recent years, researchers have developed nanowire sensors for the detection of mycotoxins.

Compared with traditional analytical methods, they have the advantages of simple operation, low detection cost, sensitivity, speed, and on-site detection. Creative Enzymes provides nanozymes and detailed schemes for the detection of mycotoxins.

Analysis of Aflatoxin B1 with Nanozyme

• Studies have shown that the ultra-high specific surface area of two-dimensional structure MnO₂ nanozymes makes it have strong adsorption capacity for TMB, and thus they have excellent oxidase-like catalytic activity for TMB oxidation. However, the presence of ascorbate oxidase effectively attenuates this process and the degree of color development increases with the concentration of ascorbate oxidase.
• Based on this, Creative Enzymes constructs a novel colorimetric immunosensor of aflatoxin B1 (AFB1) with effectively improved sensitivity by enzyme tandem catalytic amplification strategy. It has the advantages of high sensitivity, reproducibility, and stability, and provides a new approach to the analysis of trace targets based on nanozymes.

Fig. 1 Schematic illustration of MnO₂ nanozymes mediated chromatographic immunoassay on Bovine Serum Albumin-AFB1 modified magnetic beads. (Lai W, et al., 2017)

• We design and synthesize a Pt / Co / rGO nanozymes and use it as an enzyme substitute for HRP to label AFB1 antibody. Thus, we have built a highly sensitive competing electrochemical immunosensor. This method has high accuracy and precision, and it is promising for a wide range of applications in the detection of AFB1 in everyday situations.

Analysis of Ochratoxin Awith Nanozyme

• MnCo₂O₄ can be used as an oxidase to catalyze the color development of TMB due to the synergistic effect of Co³⁺ / Co²⁺ and Mn³⁺ / Mn²⁺ REDOX catalytic cycles on the crystal surface. The addition of ochratoxin A (OTA) aptamer can inhibit the electron transfer efficiency between MnCo₂O₄ and TMB, thereby weakening the degree of color reaction. However, the activity of MnCo₂O₄ is restored by the addition of OTA.

Fig. 2 MnCo₂O₄ oxidase mimic-based colorimetric assay for OTA detection.

• Based on this principle, Creative Enzymes constructs a highly sensitive and highly selective colorimetric detection method for OTA in samples.
• We also establish a method for the determination of trace OTA in samples using alkaline phosphatase and MnO₂ oxidase tandem catalytic amplification system.

Creative Enzymes provides nanozyme and veterinary drug residue detection solutions to our clients worldwide. With years of experience in biological services, our advanced platform can help our clients address the types of difficulties associated with the detection of glutathione using nanozymes. If you are interested in our services or have some questions, please feel free to contact us or make an online inquiry.