Nanozyme-Based Biosensors

Biosensors are suitable for real-time detection due to their advantages of simple operation, fast response, low cost and easy miniaturization. Sensing materials are a core component of biosensors and can determine sensitivity, response speed, linear range, and detection limits. Therefore, rational design of sensing materials is an important link in the construction of high-performance biosensors.

Nanozymes are a class of nanomaterials with intrinsic enzyme-like activity. They exhibit enzymatic reaction dynamics and catalytic mechanisms similar to natural enzymes. They have the advantages of good stability, low cost and ease of preparation, as well as obtaining more sensitive current response signals and higher detection efficiency, which offer a wide range of prospects for their practical applications. Creative Enzymes, as a leading global company, is committed to helping our clients achieve effective and successful research. We develop various nanozymes and schemes for designing biosensors.

Electrochemical Biosensors Based on Nanozymes

• In the presence of the target analyte, the biometric element of an electrochemical biosensor, such as an enzyme, antibody, or aptamer, first selectively reacts with the specific analyte, and subsequently the device converts the biometric event into a detectable electrical signal. Electrochemical biosensors play a crucial role in biological analysis.
• Creative Enzymes constructs a novel electrochemical cell sensor by introducing the Au / Pd / Mo nanozyme to determine circulating tumor cells. The sensor has promising analytical performance and can provide a promising platform for early detection of tumor cells.

Fig. 1 Schematic diagram of the electrochemical biosensor based on Au / Fe₂O₃ nanozyme for exosome detection. (Boriachek K, et al., 2019)

• We also synthesize Au / Fe₂O₃ with peroxidase-like activity and superparamagnetic properties for exosome detection. The immunosensor is constructed by incubating Au / Fe₂O₃ nanozymes conjugated to electrodes, which catalyze the oxidation of TMB and H₂O₂ and emit an electrochemical signal.

Colorimetric Biosensors Based on Nanozymes

• Creative Enzymes synthesizes Cu / Fe₃O₄ nanozymes with peroxidase activity. With modifications and compounds, we establish colorimetric biosensors to detect H₂O₂, glutathione, dopamine, and other substances studied by the easy-to-operate colorimetry method.
• We also synthesize carbon quantum dots (QDS) with peroxidase mimetic activity, and thus establish a colorimetric biosensor for the detection of fluoride ions in aqueous solution with high selectivity and sensitivity. It can be used for real-time detection in practice.

Fluorescent Biosensors Based on Nanozymes fluorescent

• Fluorescence sensing methods have the advantages of high sensitivity, good selectivity, simple operation, real-time detection, and good portability, which have been widely used in many fields such as life science, medical imaging, biosensing analysis, and so on.

Fig. 2 A colorimetric and fluorescent dual-channel method for ALP activity detection is proposed. (Pengjuan N, et al., 2019)

• We develop a fluorometric assay for alkaline phosphatase (ALP) activity based on bifunctional peroxidase activity, the fluorescence reaction occurs in bovine serum albumin and gold nanozymes. The detection is sensitive and the results are satisfactory.

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