Enzymes are the indispensable core substance of life. Gene editing, stem cell technology, targeted drugs and many other key technologies and products in life sciences are inseparable from enzymes. With the continuous development of modern technology, scientists' understanding of enzymes has become more in-depth, and enzyme synthesis plays an important role in promoting life science research, drug discovery, industrial production, environmental protection, and energy conservation.

Creative Enzymes has a wealth of research and service experience in the field of enzyme synthesis. From enzyme extraction to isolation, and enzyme biosynthesis to enzyme chemical synthesis, we provide important guarantees to ensure the accuracy, efficiency, high activity, and stability of enzyme synthesis.

Enzyme Extraction and Isolation

We offer enzyme extraction and isolation, which is the process of extracting enzymes from complex organisms and passing them through different separation and purification techniques to finally obtain highly efficient and pure enzyme extracts. Enzyme extraction and isolation, therefore, occupy an important position in enzyme synthesis. We utilize advanced enzyme extraction and isolation techniques to provide tools to ensure that highly stable, pure, and active enzymes are obtained.

Enzyme Biosynthesis

In enzyme biosynthesis, we utilize genetic engineering, such as gene editing technology, which can effectively improve the ability of microorganisms to produce enzymes by fermentation through targeted and accurate modification of microorganisms' genes. In addition, we utilize evolutionary experiments to obtain mutant strains with targeted properties by introducing mutations into genes edited for biocatalysis i.e., through the use of various mutation methods, and ultimately change the performance of the enzyme through continuous mutation and screening. Currently, we synthesize enzymes mainly through the following experimental protocols.

• Microbial fermentation for enzyme production.
• Plant cell culture enzyme production.
• Animal cell culture enzyme production.

Chemical Synthesis of Enzymes

• Design. The target enzyme's amino acid sequence must first be designed. This method entails altering or developing a completely new enzyme based on the sequence of an existing enzyme.
• Chemical Synthesis. Chemical synthesis techniques like liquid phase synthesis or solid phase synthesis are frequently used in enzyme synthesis processes. To create the amino acid sequence and guarantee appropriate target enzyme folding, we use chemical synthesis.
• Folding and Modification. An enzyme's amino acid sequence must be folded and changed after it has been created in order for the enzyme to operate. This may involve supplying the required cofactors or coenzymes as well as directing the enzyme to fold correctly under particular circumstances.
• Activity Testing and Optimization. To ensure good catalytic activity, we shall test the produced enzyme's activity at this stage. To ensure high catalytic efficiency and good stability of the enzyme, we will then further enhance the performance of the first synthetic enzyme created.

Our Service Workflow

Creative Enzymes has many years of research experience in the field of enzyme synthesis. Based on our advanced technology platform and experienced team of experts, we offer a wide range of effective solutions for specific enzyme synthesis according to customer needs. If you are interested in us, please feel free to contact us.