Eliminating Drug-Resistant Bacteria with Nanozymes

Currently, drugs used in antimicrobial therapy are mainly divided into antibiotics and synthetic antimicrobials, however, more and more bacteria are becoming resistant to antibiotics. The solution to the problem of bacterial resistance is to modify the structure of conventional antibiotic molecules to prepare various antibiotic derivatives, but this still does not meet the need to eliminate resistant bacteria. Therefore, there is an urgent need to develop novel antimicrobial agents to address multi-drug resistance.

Nanozymes can rapidly kill a wide range of bacteria by catalyzing the production of ROS, releasing metal ions, and combining therapies. This suggests that nanozymes have a unique advantage over existing antimicrobial agents. Creative Enzymes provides types of nanozymes for drug-resistant bacteria, helping our customers worldwide in the field of the nanozymes.

Catalyzing and Generating Reactive Oxygen Species for Eliminating Bacteria

• Nanozymes with oxidoreductase activity can kill and eliminate bacteria in vivo by regulating the reactive oxygen species (ROS) (¹O₂, ·OH, O₂^-).
• Creative Enzymes constructs Fe₃O₄ nanozymes with high POD activity, which can catalyze H₂O₂ to produce more toxic hydroxyl radical (·OH) and kill most E. coli.

Fig. 1 Fe₃O₄ nanozymes kill bacteria by generating ROS.

• We develop Tb₄O₇ nanozymes with OXD activity, which can directly catalyze the bacterial endogenous substance ascorbic acid to generate H₂O₂ and hydroxyl radicals (·OH). They can completely suppress the growth of E. coli at certain concentrations.
• We also establish Cu₂WS₄ nanozymes exerting POD and OXD activity, to kill staphylococcus aureus by catalyzing the generation of O2 -H₂O₂-·OH in a cascade.

Releasing Metal Ions for Eliminating Bacteria

• Moreover, the release of metal ions can also kill bacteria. Many metal ions are bactericidal, including silver, iron, manganese, copper, zinc, mercury, and aluminum, with copper and silver being the most widely used in antibacterial applications.

Fig. 2 Nanozymes kill bacteria by releasing metal ions.

• Creative Enzymes constructs a copper / carbon nanozymes to release Cu²⁺, which can cause cell membrane damage, lipid peroxidation, and DNA degradation. It ultimately causes the death of Gram-negative bacteria.
• We also establish Ag / Fe₃O₄ nanozymes, which release Ag⁺ and exert antibacterial effects through multiple physicochemical mechanisms. They have significant long-term antimicrobial effects against both Gram-negative and Gram-positive bacteria.

Combination Therapy for Eliminating Bacteria

• Creative Enzymes synthesizes nitrogen / carbon nanozymes to further enhance POD activity under laser irradiation. Locally generated heat acts synergistically with the chemical action of ·OH to kill multi-resistant Staphylococcus aureus.
• We also develop a Pd / Pt nanozyme, whose catalytic activity combined with photosensitizer activation to generate large amounts of ROS to kill bacteria under ultrasound.

Creative Enzymes offers drug-resistant bacteria based on nanozymes with customized delivery strategies and precise design. Our state-of-the-art technology platform helps our clients solve problems they may encounter in research based on decades of experience. If you are interested in our services or have some questions, please feel free to contact us or make an online inquiry.