Clearing Biofilms with Nanozymes

Bacterial biofilms are aggregated membrane-like substances surrounded by a polysaccharide matrix, fibrin and lipoproteins secreted by bacteria, and are protective barriers formed by bacteria to adapt to their natural environment. Statistics show that 65 percent of human bacterial infections are caused by bacterial biofilms, such as dental caries and gingivitis, cystic fibrosis of the lungs, urinary tract infections, respiratory tract infections, recurrent wound infections, and infections caused by implanted medical devices. However, many macromolecular antimicrobials cannot pass through biofilms, which becomes a difficult problem in the elimination of bacteria.

Because nanozymes are at the nanoscale, they can not only penetrate into the interior of the biofilm, but also destroy the main components of the biofilm by generating ROS in situ. Creative Enzymes provides types of nanozyme for the removal of biofilms, helping our customers worldwide in this field. We customize featured services to the needs of our customers.

Clearing Streptococcus Mutans Biofilms with Nanozymes

• Streptococcus mutans biofilm is a typical representative of cariogenic biofilm. Although the percentage of this bacteria in the entire oral flora is not high, it is a major contributor to dental caries.

Fig. 1 Clearing a Streptococcus mutans biofilm using nanozymes.

• Creative Enzymes develops ferric oxide nanozymes that catalyze the efficient degradation of biomolecules such as DNA, proteins, and polysaccharides by hydrogen peroxide under acidic conditions.
• The nanozymes catalyze the degradation of biofilm matrix components, thereby destroying the structure of biofilm matrix and efficiently killing internal bacteria.

Clearing Staphylococcus Aureus Biofilms with Nanozymes

• Creative Enzymes assembles passivated gold nanozymes containing tetravalent cerium onto the surface of magnetic Fe₃O₄ / silica core-shell colloidal particles. The compound has DNase-like activity and can shear-degrade DNA molecules.
• The nanozyme composite can degrade extracellular DNA in Staphylococcus aureus biofilm matrix, which can effectively prevent more than 90% of bacterial adhesion and inhibit early biofilm growth. At the same time, it can degrade eDNA in mature biofilms, destroying the integrity of the biofilm structure.

Clearing Gram Vaginalis Biofilms with Nanozymes

• We also establish that FeS nanozymes possessing POD enzymatic activity.
• The nanozymes release polysulfide can quickly enter the Gram vaginalis with thin bacterial wall and inhibit GLK to block the glycolytic pathway. On the other hand, the release of ferrous iron, in conjunction with the polysulfur-mediated blocking effect of energy metabolism, leads to bacterial ferrocytosis.

Creative Enzymes provides services covering antiviral devices with enzymes to meet your research needs. With years of experience in the biological industry, our specialized platform is able to help our clients solve these types of difficulties. If you are interested in our services or have some questions, please feel free to contact us or make an online inquiry.