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Iodonitrotetrazolium chloride
Cat No.
CSUB-0341
Applications
Electron acceptor for the colorimetric assay of various dehydrogenasesIodonitrotetrazolium (INT) is a tetrazolium dye precursor that forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. INT is used as an electron acceptor for the colorimetric assays of: lactate dehydrogenase, xanthine dehydrogenase, lactyl-CoA dehydrogenase, succinate dehydrogenase, BBM II ketolisomerase, histidinol dehydrogenase and diverse other hydrolases.
Enzyme Commission Number
205-676-2
CAS No.
146-68-9
Molecular Weight
505.70
Synonyms
2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride; p-Iodonitrotetrazolium Violet; INT
Molecular Formula
C19H13ClIN5O2
MDL Number
MFCD00011961
Solubility
methanol: water (1:1): soluble 50 mg/mL, very faintly turbid, very deep yellow (hot)
PubChem Substance ID
24881028
mp
240 °C (dec.)(lit.)
Beilstein Registry Number
4093224
Substrates
Lactyl-CoA Dehydrogenase
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